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Pregnenolone is a naturally occurring metabolite of cholesterol that acts as a precursor to all adrenal cortical steroids, including progesterone, testosterone, dehydroepiandrosterone, dihydrotestosterone, aldosterone, cortisol, and estradiol. The body produces pregnenolone from cholesterol in the mitochondria through the cytochrome P450 conversion system. The brain requires pregnenolone, producing it and also absorbing more from the blood.